Object: Maizhuninujia as the selection list of Chinese classic prescription of uyghur medicine. This prescription comes from the classic ancient book of national medicine of the late Ming Dynasty, Baidiyi Medicinal Book. The prescription consists of Phyllanthus emblica , Terminalia chebula Retz, Cuscuta chinensis, Lavandula angustifolia Mill, Rhizoma Polypodiodis Nipponicae, which has the role of clearing abnormal splenic fluid quality, blocking and invigorating brain, and is used to treat neurasthenia caused by abnormal splenic fluid quality. The syndrome of amnesia and depression (Malihuria) has obvious characteristics and advantages. The subject through the quality evaluation of collected medicinal materials or decoction pieces, screened out qualified batches; Then, according to the processing technology of two kinds of medicinal materials obtained from ancient books of uyghur medicine, the quality study of 10 batches was established. The intermediate powder process was summarized according to the smashing characteristics and mixing characteristics of each medicine flavor in Maizhuninujia prescription, and the honey refining process was reperformed according to the traditional honey refining process of TCM. The single factor experiment and orthogonal experiment were designed to mix the intermediate powder and honey to obtain the preparation process, To study the effect of Maizhuninujia on chronic unpredictable mild stress mode, lwhich provided scientific basis for the classic famous prescription of Maizhuninujia prescription to buy the clinical market.
Method: (1) According to the four General rules of the 2020 edition of Chinese Pharmacopoeia, the Processing Standards of Xinjiang Uygur Autonomous Region for Chinese Herbal Medicine Decoction Pieces and the existing standards of the Department of Herbal Medicine, quality inspection was conducted on the identification, impurities, moisture, total ash, acid insoluble ash, extract, content determination and other inspection items of all batches of medicinal materials collected from Maizhuninujia a prescriptions. Check the standard to determine whether the medicine is qualified; (2) The processing technology of P. embalm was optimized, and the quality of 10 batches of P. embalm was studied, and the characters, moisture, total ash, acid insoluble ash, extract and content of the medicinal materials were determined. At the same time, the quality of 10 batches of auxiliary milk used for processing P. embalm was checked. (3) In the experiment, the grinding characteristics of each flavor in Maizhuninujia prescription were determined by individual crushing, followed by partial crushing and whole crushing to determine the optimal grinding process and formulate the pretreatment method of the medicinal materials. After that, the RSDS of the contents of gallic acid, hypericin and rosmarinic acid in the intermediate powder under different mixing times were compared by an efficient mixer to select the optimal mixing process. (4) According to the text description of honey refining in traditional process of uyghur medicine, the yield, relative density and 5-hydroxymethylfurfural content were used as indicators to investigate the honey refining temperature, honey refining time and other factors to obtain the best process, and the honey refining process was confirmed after three batches of verification; (5) Single factor and orthogonal experiments were designed to study the preparation technology. The content, relative density and uniformity of gallic acid, 5-hydroxymethylfurfural and hypericin were taken as indexes, followed by A, B, C, D and other factors, and the optimal process was verified. (6) To conduct pharmacodynamic study of Maizhuninujia on chronic unpredictable mild stress mice model, conduct weight monitoring, behavioral investigation, cytokine and HE staining determination and analysis, and preliminarily establish efficacy evaluation.
Results: (1) Quality inspection of medicinal materials: A total of 64 batches of five medicinal materials of the formulation were collected, including 16 batches of Radix embalmiae, 16 batches of Xanthophylla, 10 batches of tiger herb, 9 batches of aqua keel, and 13 batches of lavender medicinal materials. Sixty-one batches were qualified through quality inspection, the content determination of Radix embalmiae in one batch did not meet the requirements, and the inspection items of acid insoluble ash in two batches of lavender did not meet the requirements. (2) Optimize the processing technology of Camellia japonicum in the laboratory to change the ratio of material to liquid, establish and discuss the quality inspection of 10 batches of gun products, and carry out the quality inspection of 10 batches of auxiliary milk for processing, all qualified; (3) According to the grinding characteristics of each flavor in Maizhuninujia prescription, the grinding process was summarized, the pretreatment method of the medicinal materials was formulated, and the whole method of grinding was selected according to the powder yield. After comparing the RSDS of gallic acid, hypericin and rosmarinic acid in the intermediate powder with different mixing time, the optimal mixing time was 45 minutes in the high efficiency mixer. The preparation process of the intermediate powder was obtained. (4) Honey refining research: The best honey refining process was heated at 80℃ for 30 minutes. The color of honey was darker than that of raw honey. The content of 5-hydroxymethylfurfural met the standard (< 40 μg/g). (5) Study on the process of Maizhuninujia preparation: the best process is A 100, B 40, C 0.25, D 1:5 cooling to room temperature. (6) Pharmacodynamic results: Maizhuninujia medium and high dose groups have certain antidepressant treatment effect on the chronic unpredictable mild stress mouse model, and can improve the behavior of mice. The antidepressant effect is related to the upregulation of monoamine neurotransmitters 5-HT, DA and neurotrophic factors.
Conclusion: The qualified rate of Maizhuninujia formula is high. After processing, the toxicity of Phyllanthus emblica Land Terminalia chebula Retz was significantly reduced, and the processing technology, intermediate preparation technology, honey refining technology and preparation technology were stable, Maizhuninujia has antidepressant effects; Can provide the basis for the purchase of subsequent Maizhuninujia standards.
谷歌
火狐
